中文摘要：

目前學(xué)界對(duì)大腦受損血管修復(fù)過(guò)程中的細(xì)胞調(diào)控機(jī)制尚不明確，針對(duì)糖尿病等存在血管病變高危因素人群的相關(guān)研究尤為匱乏。本研究剖析了腦組織固有小膠質(zhì)細(xì)胞與浸潤(rùn)性巨噬細(xì)胞在破損腦微血管修復(fù)進(jìn)程中的作用。

英文摘要：

The cellular events that dictate the repair of damaged vessels in the brain, especially in those with vascular risk factors such as diabetes, is poorly understood. Here, we dissected the role of resident microglia and infiltrative macrophages in determining the repair of ruptured cerebral microvessels. Using in vivo time-lapse imaging, gene expression analysis, and immunohistochemistry, we identified a unique population of phagocytic Galectin 3 (Gal3) expressing macrophages, distinct from resident microglia, which infiltrated and aggregated at the site of injury in diabetic mice and were associated with the elimination of microvessels. Depletion of these infiltrative macrophages in diabetic mice attenuated phagocytic activity and prevented the loss of blood vessels after injury. These findings highlight a previously unknown role for infiltrative Gal3 expressing macrophages in promoting vessel elimination after brain injury and provide impetus for future studies to determine whether depleting these cells can facilitate vascular repair in at risk populations.

論文信息：

論文題目：Invasion of phagocytic Galectin 3 expressing macrophages in the diabetic brain disrupts vascular repair

期刊名稱(chēng)：Science Advances

時(shí)間期卷：Vol 7, Issue34(2021)

在線時(shí)間：2021年8月18日

DOI: 10.1126/sciadv.abg2712

產(chǎn)品信息：

貨號(hào)：CP-005-005

規(guī)格：5ml+5ml

品牌：Liposoma

產(chǎn)地：荷蘭

名稱(chēng)：Clodronate Liposomes&Control Liposomes

辦事處：靶點(diǎn)科技

Clodronate Liposomes氯膦酸鹽脂質(zhì)體靜脈注射，清除外周巨噬細(xì)胞。荷蘭Liposoma巨噬細(xì)胞清除劑ClodronateLiposomes見(jiàn)刊于Science Advances：吞噬型半乳糖凝集素 3 陽(yáng)性巨噬細(xì)胞浸潤(rùn)糖尿病小鼠腦組織會(huì)阻礙血管修復(fù)。

Liposoma巨噬細(xì)胞清除劑Clodronate Liposomes氯膦酸二鈉脂質(zhì)體清除巨噬細(xì)胞的材料和方法：

In vivo macrophage depletion

For drug intervention experiments, mice received intravenous CLR (50 mg/kg; Liposoma B.V.) 2 days before CMB induction, on the day of CMB induction, and 2 days later. This treatment regimen ensured sustained depletion of peripheral macrophages. Diff-Quik histological staining confirmed depletion of circulating immune cells in treated animals.

藥物干預(yù)實(shí)驗(yàn)中，于腦微出血造模前 2 天、造模當(dāng)日及造模后 2 天，對(duì)小鼠尾靜脈注射氯膦酸二鈉脂質(zhì)體（CLR，給藥劑量 50 mg/kg，廠商：Liposoma B.V.）。該給藥方案可實(shí)現(xiàn)外周巨噬細(xì)胞的持續(xù)性清除。經(jīng)迪夫快速（Diff-Quik）組織染色驗(yàn)證，給藥小鼠外周循環(huán)免疫細(xì)胞已被有效清除。

巨噬細(xì)胞清除材料和方法文獻(xiàn)截圖：吞噬型半乳糖凝集素 3 陽(yáng)性巨噬細(xì)胞浸潤(rùn)糖尿病小鼠腦組織會(huì)阻礙血管修復(fù)